Talk Title: In Situ Mapping of Cancer Mechanisms and Microenvironments via Pooled Perturbations Abstract: We developed CRISPRmap, a novel in situ barcode readout approach with concurrent multiplexed immunofluorescence and in situ RNA detection. CRISPRmap reduces both dependency on third party proprietary sequencing reagents and assay cost. We conducted a multi-omic base-editing screen in a breast […]

Talk Title: Dissecting gliomas by single-cell and spatial genomics Abstract: In my lecture, I will discuss recent efforts we have undertaken in the lab to characterize the architecture of glioblastoma and its temporal evolution after standard of care therapy by single-cell and spatial genomics. I will also discuss novel approaches that we have developed to […]

Talk Title: Mapping transcriptional responses to cellular perturbation dictionaries with RNA fingerprinting