Talk Title: Mapping transcriptional responses to cellular perturbation dictionaries with RNA fingerprintingRead More

Talk Title: Dissecting gliomas by single-cell and spatial genomics Abstract: In my lecture, I will discuss recent efforts we have undertaken in the lab to characterize the architecture of glioblastoma and its temporal evolution after standard of care therapy by single-cell and spatial genomics. I will also discuss novel approaches that we have developed to...Read More

Talk Title: In Situ Mapping of Cancer Mechanisms and Microenvironments via Pooled Perturbations Abstract: We developed CRISPRmap, a novel in situ barcode readout approach with concurrent multiplexed immunofluorescence and in situ RNA detection. CRISPRmap reduces both dependency on third party proprietary sequencing reagents and assay cost. We conducted a multi-omic base-editing screen in a breast...Read More

For invited lecture recording, please click here Talk Title: Quantifying tumor heterogeneity in space and time Abstract: Tumors are heterogeneous mixtures of cancerous and non-cancerous cells that interact in distinct spatial niches and that evolve over time and in response to treatment. Recent spatial transcriptomics technologies measure RNA expression at thousands of locations in a...Read More

For seminar recording, please click here Talk Title: Single-cell Spatial Omics Journey to Signaling and Metabolism of Immunity, Cancer, and Aging Abstract: The spatial organization of cells in tissues and subcellular networks provides a quantitative metric for determining health and disease states. Single-cell analyses of molecular profiles with in-situ detection methods dissect spatial heterogeneity of...Read More

Talk Title: mtDNA: the Cinderella of cancer genetics Abstract: Mutation of mitochondrial DNA (mtDNA) can have broad impacts on cell metabolism and organism physiology, and mutation of mtDNA is among the most common somatic alterations to the tumour genome. Mutation of mtDNA is an established cause of multiple hereditary metabolic diseases, with metabolic impacts on...Read More

To view the seminar recording, please click here Talk Title: Statistical approaches for deciphering gene programs in cancer cells across studies and mutational signatures across ancestries Abstract: Single-cell assays provide valuable insights into the biological processes active in cancer cells within and across tumor samples. However, the majority of single cell RNA-seq (scRNA-seq) studies only...Read More

To view the seminar recording, please click here Talk Title: Deciphering tissue pathogenesis with spatial sequencing Abstract: Spatial and molecular characteristics determine tissue function, yet high-resolution methods to capture both concurrently are lacking. In recent years, we developed high-definition spatial transcriptomics and multi-omics technologies, which captures RNA, protein information or microbiota from histological tissue sections...Read More